Lindsley Register was born and raised in Blue Ridge Mountains of Virginia, United States. She was brought up with two older brothers and a younger sister. She went to Liberty University, and it was not until Register ditched a major in Teaching English as a Second Language during college that she started to take acting seriously.

During her first year after graduating from college, Register received a costarring role in episode 404 of House of Cards, portraying one of the protesters opposite to Kevin Spacey. This would help her get several more roles in indie films and other notable shows, such as The Haves and the Have Nots, where she plays as Karen, and Outcast.[2] Her first series regular role came in the new History channel series Six. Most recently, she has become well known for her role as Laura in The Walking Dead. Register joined The Walking Dead cast during season 7 of the show, and has been a part of the show ever since. In a YouTube video posted during the production of season 8, Register revealed how being called up to The Walking Dead was one of the most terrifying, yet exciting challenges and moments in her career.